Defeat the indicated Boss as an Autobot (to unlock an Autobot Boss) or a Decepticon (to unlock a Decepticon Boss) in the Arena to unlock them as a playable character.
After tutorial cinematic is over, enter the first room. Talk to Ratchet and Air Raid, then turn right. Find the three computer monitors along the wall. Walk up to the middle monitor that is under the spotlight. You wil have a conversation with a person named Kup.
In the "code input" option, unlock the following characters entering these codes:
Enter 99871 as a code to unlock Ramjet in Multiplayer mode.
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Beating bosses in the arena, unlock these bosses as playable characters. Note: You must play the boss´ stage with autobots to unlock the autobot boss, and with decepticons to unlock the decepticon boss.